Data from: Evidence for polyphosphate accumulating organism (PAO)-mediated phosphorus cycling in stream biofilms under alternating aerobic/anaerobic conditions

Metadata Updated: November 10, 2020

Phosphorus availability often limits primary production in freshwater ecosystems and excessive P inputs promote accelerated eutrophication. Microbial mechanisms may control O2-dependent uptake/release of P in stream sediments and biofilms, but specific organisms responsible for these cycles have not been identified. Polyphosphate Accumulating Organisms (PAOs) are purposely enriched in treatment plants to remove P from wastewater. PAOs release P under anaerobic conditions and take it up under aerobic conditions. It is hypothesized that alternating aerobic/anaerobic conditions promote patterns of P uptake/release similar to those attributed to PAOs in wastewater treatment. Intact, native stream biofilms were collected in the Cascadilla Creek Watershed in Tompkins County, New York, and subjected to laboratory treatments to impose conditions similar to what may occur because of diel oxygenic and respiratory cycles: 1) continuous sparging with air and 2) alternate sparging with air or anaerobic gas (20∶80% by volume CO2∶N2). PO43−, Ca, Mg, total Mn, K, Fe2+, and total S (TS) concentrations in the water were monitored and total P (TP) and polyphosphate (polyP) concentrations in the biofilms at the start and end of the experiment. Microscopy and polymerase chain reaction (PCR) were used to quantify the percentage of cells with stored intracellular polyP and to test for known PAO genes, respectively. This dataset comprises the data, analysis scripts, and visualization scripts for the paper by Saia et al. entitled 'Evidence for polyphosphate accumulating organism (PAO)-mediated phosphorus cycling in stream biofilms under alternating aerobic/anaerobic conditions. Complete dataset description including information on associated journal article, data, and data analysis R scripts can be found at https://github.com/sheilasaia/paper-p-cycling-in-stream-biofilms/tree/v1....

Access & Use Information

Public: This dataset is intended for public access and use. License: Creative Commons CCZero

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Dates

Metadata Created Date November 10, 2020
Metadata Updated Date November 10, 2020

Metadata Source

Harvested from USDA JSON

Additional Metadata

Resource Type Dataset
Metadata Created Date November 10, 2020
Metadata Updated Date November 10, 2020
Publisher Agricultural Research Service
Unique Identifier Unknown
Maintainer
Identifier 1757fec5-0835-4322-853f-d0b8f1b3b0ae
Data Last Modified 2019-08-23
Public Access Level public
Bureau Code 005:18
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
License https://creativecommons.org/publicdomain/zero/1.0/
Program Code 005:040
Source Datajson Identifier True
Source Hash 1f911d21bffac5573239eeb6f5a22c813d08f6a1
Source Schema Version 1.1
Spatial POINT (-76.507415771484 42.451708009821)

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