Detection of Seagrass Pathogens Using Environmental DNA (eDNA), North Pacific, 2016-present

Metadata Updated: November 12, 2020

This data set is comprised of one table with sampling information and NCBI BioProject accession numbers for sequence information of this amplicon-based study targeting Labyrinthula, Phytophthora, and Halophytophthora of known pathogenic and non-pathogenic [to eelgrass (Zostera marina)] strains from eDNA samples. eDNA samples included water, sediment, and eelgrass from Izembek Lagoon and cloacal swabs from waterfowl hunted near Cold Bay, Alaska. Replicate samples and multiple sampling dates of the same location were included. Highly conserved primers which could differentiate species of interest were developed for four portions of mtDNA genes (5.8S, 18S, ITS, and COI). The reference list and conserved primer sets to identify species present were developed using publicly available data https://www.ncbi.nlm.nih.gov/genbank/

Access & Use Information

License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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Dates

Metadata Date April 27, 2020
Metadata Created Date November 12, 2020
Metadata Updated Date November 12, 2020
Reference Date(s) April 27, 2020 (publication)
Frequency Of Update irregular

Metadata Source

Harvested from DOI Open Data

Additional Metadata

Resource Type Dataset
Metadata Date April 27, 2020
Metadata Created Date November 12, 2020
Metadata Updated Date November 12, 2020
Reference Date(s) April 27, 2020 (publication)
Responsible Party U.S. Geological Survey, Alaska Science Center (Point of Contact)
Contact Email
Guid
Access Constraints Use Constraints: It is requested that the authors and the USGS Alaska Science Center be cited for any subsequent publications that reference this dataset., Access Constraints: None
Bbox East Long -162.7229
Bbox North Lat 55.27011
Bbox South Lat 55.21065
Bbox West Long -162.8974
Coupled Resource
Frequency Of Update irregular
Licence Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey, no warranty expressed or implied is made regarding the display or utility of the data for other purposes or on all computer systems, nor shall the act of distribution constitute any such warranty. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.
Metadata Language
Metadata Type geospatial
Progress completed
Spatial Data Service Type
Spatial Reference System
Spatial Harvester True
Temporal Extent Begin 2016-01-01
Temporal Extent End 2019-01-01

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