Low-power, Confocal Imaging of Protein Localization in Living Cells (7214-150), Phase I

Metadata Updated: November 12, 2020

The proposed technology genetically labels intracellular structures and visualizes protein interactions in living cells using a compact, confocal microscope with solid-state laser illumination. The proposed innovation will allow advanced study of cellular biology in microgravity environments. The recently introduced reef coral proteins fluoresce over a broader range of wavelengths than GFP and its derivatives. This allows simultaneous fluorescence labeling of three, and possibly four, cellular constituents simultaneously. The proposed confocal microscope will be based entirely on compact, low-power solid-state laser technology and will allow high-resolution imaging of structures within living cells in three colors simultaneously at standard video frame rates. As part of this innovation we will develop a 594 nm solid state laser for improved resolution of HcRed, the most red-shifted of the reef coral proteins. In Phase I we will set up a biological model that incorporates three different protein fusions. Phase I microscopy studies will determine the specifications for spectral and spatial resolution for the Phase II device. That Phase II device will comprise a compact system that uses low-power solid-state laser illumination. The device will have broad applicability for space cell biology research and also for terrestrial-based cell biology research.

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Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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Metadata Created Date November 12, 2020
Metadata Updated Date November 12, 2020

Metadata Source

Harvested from NASA Data.json

Additional Metadata

Resource Type Dataset
Metadata Created Date November 12, 2020
Metadata Updated Date November 12, 2020
Publisher Space Technology Mission Directorate
Unique Identifier Unknown
Identifier TECHPORT_5412
Data First Published 2004-07-01
Data Last Modified 2020-01-29
Public Access Level public
Bureau Code 026:00
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Metadata Catalog ID https://data.nasa.gov/data.json
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Homepage URL https://techport.nasa.gov/view/5412
Program Code 026:027
Source Datajson Identifier True
Source Hash 609c34eb4c2614c6e8967b44291885cdc130dd69
Source Schema Version 1.1

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